Reconstitution of Epstein-Barr nuclear antigen 1 (EBNA1)-mediated plasmid segregation in budding yeast requires human EBP2

  • 0.94 MB
  • English
National Library of Canada , Ottawa
SeriesCanadian theses = -- Thèses canadiennes
The Physical Object
Pagination2 microfiches : negative.
ID Numbers
Open LibraryOL19072617M
ISBN 10061263132X

Fig 1. T-cell responses against Epstein-Barr virus nuclear antigen 1 (EBNA-1), latent membrane protein 2 (LMP-2), and p54 in healthy donors. Mean frequency of antigen-specific T cells against EBNA-1 is compared with early antigen p54 and LMP-2 (n = 8).

Antigen-specific T lymphocytes were found in all seropositive by: EBV nuclear antigen 1 (EBNA-1) as an antigen to generate EBV-specific CD4 and CD8 T cells from EBV-seropositive donors in a time-saving and simple procedure without any in vitro expansion steps. This approach is based on the infusion of small amounts of donor T cells and their subsequent in vivo expansion to mount an.

Epstein-Barr virus (EBV) is a human gamma herpes virus that is best known for being the causative agent of infectious mononucleosis in man. A fascinating feature of this virus is its ability to persist in the host and it is estimated that more than 95% of adults are carriers of the virus.

Importantly, EBV can transform latently infected primary cells from healthy individuals into cancerous. Epstein-Barr Nuclear Antigen 1 (EBNA1)-dependent Recruitment of the latent origin of Epstein-Barr virus, provides an excellent model system for the study of initiation on the host chromosomes because it is known to depend on prereplicative complex factors, including Orc and Mcm.

Here, we show that Orc is recruited selectively at the Cited by:   Introduction. Epstein-Barr virus (EBV) is a latent human virus that infects >90% of the adult healthy carriers, it is controlled in the latent state by the immune system, especially by EBV-specific T cells 2, the first 6 months after hematopoietic stem cell transplantation (HSCT), while immune reconstitution is not fully achieved and immunosuppressive drugs are used to Cited by: 6.

One viral protein, Epstein–Barr nuclear antigen 1 (EBNA1), and one cis‐acting viral DNA fragment, oriP, are required for the replication and partitioning of the viral episomes. OriP includes two essential elements termed the family of repeats (FR) and the dyad symmetry (DS) element, that contain twenty Reconstitution of Epstein-Barr nuclear antigen 1 book four EBNA1 binding sites respectively.

Its infectivity was assayed by the induction of the EBV-determined nuclear antigen (EBNA) and early antigen (EA) [14,15]. EBV-B (1: 20 dilution) induced 15% EBNA positive cells 48 h after infection of the Ramos line.

EBV-P (1: dilution). EA IgG (Early Antigen) – this is the most important test and it’s often missed. If your doctor skips this one you won’t know your true EBV status.

EBNA IgG (Epstein Barr Nuclear Antigen) – Indicates a history of infection in Reconstitution of Epstein-Barr nuclear antigen 1 book cases.

This tells the story of the long term status of the virus. Epstein-Barr nuclear antigen (EBNA) antibodies are directed against proteins that the virus uses to survive and replicate its DNA after it enters into a dormant phase [20, 2, 21].

EBNA antibodies are only detectable weeks after the onset of symptoms, after mono has run its course and the virus is no longer active. Epstein Barr virus nuclear antigen 1 (EBNA-1) peptides recognized by adult multiple sclerosis patient sera induce neurologic symptoms in a murine model Author links open overlay panel Neelakshi R.

Jog a Micah T. McClain a b c 1 Latisha D. Heinlen a Timothy Gross a Rheal Towner d Joel M. Guthridge a b Robert C. Axtell a Gabriel Pardo a John B. 1. Introduction.

Epstein-Barr virus (EBV), a species also known as Human gammaherpesvirus 4, belongs to the Herpesviridae family, subfamily Gammaherpesvirinae, of genuswhich most commonly manifests clinically as infectious mononucleosis (IM), has a double stranded linear DNA genome, encased within an icosahedral capsid.

Epstein–Barr virus (EBV) immortalizes host cells as part of its latent mode of infection. As a result of this ability to promote cell proliferation and survival, EBV infection contributes to the development of several kinds of B-cell lymphomas and epithelial tumours.

The EBV Epstein–Barr nuclear antigen 1 (EBNA1) protein is the only EBV protein expressed in all EBV-associated tumours and. INTRODUCTION. Epstein–Barr virus (EBV) genomes are maintained in latently infected human cells as double-stranded, circular DNA episomes in the host cell nucleus ().The viral episomes are replicated once per cell cycle and are efficiently partitioned to daughter cells during cell division (reviewed in 2).One viral protein, Epstein–Barr nuclear antigen 1 (EBNA1), and one cis-acting viral.

Multiple antigen peptides derived from Epstein‐Barr virus (EBV)–encoded Epstein‐Barr nuclear antigen 1 (EBNA‐1) were synthesized, substituting the arginines with citrulline, and were used to screen RA sera.

Anti–cyclic citrullinated peptide antibodies were purified by affinity chromatography and tested on a panel of in vitro. Diagnosis of Epstein-Barr virus (EBV) infection is based on clinical symptoms and serological markers, including the following: immunoglobulin G (IgG) and IgM antibodies to the viral capsid antigen (VCA), heterophile antibodies, and IgG antibodies to the EBV early antigen-diffuse (EA-D) and nuclear antigen (EBNA-1).

The use of all five markers results in 32 possible serological patterns. V Icheva, S Kayser, D Wolff, etal: Adoptive transfer of Epstein-Barr virus (EBV) nuclear antigen 1–specific T cells as treatment for EBV reactivation and lymphoproliferative disorders after allogeneic stem-cell transplantation J Clin Oncol 39 – 48, Link, Google Scholar:   Sternås L, Middleton T, Sugden B.

The average number of molecules of Epstein-Barr nuclear antigen 1 per cell does not correlate with the average number of Epstein-Barr virus (EBV) DNA molecules per cell among different clones of EBV-immortalized cells. J Virol. ; 64 (5)– [PMC free article]. Epstein-Barr virus (EBV), also known as human herpesvirus 4, is a gamma herpes virus that occurs only in humans.

Laboratory testing can help distinguish whether someone is susceptible to EBV infection or has a recent or past infection. Healthcare providers can test for antibodies to the following EBV-associated antigens.

Download Reconstitution of Epstein-Barr nuclear antigen 1 (EBNA1)-mediated plasmid segregation in budding yeast requires human EBP2 PDF

Richard R. Burgess, in Methods in Enzymology, An example of using PEI to precipitate a basic protein bound to DNA. Recently (Duellman and Burgess, ), in trying to purify the very basic protein Epstein–Barr virus nuclear antigen 1 (EBNA1) expressed in E. coli, we carried out a PEI precipitation test at M NaCl to see if we could precipitate the nucleic acid and acidic.

EBNA1: The Sole Trans-Acting Element of EBV Required for Its Plasmid Replicon. Epstein-Barr Nuclear Antigen 1 (EBNA1) is the viral initiator protein of EBV (Lupton and Levine ; Yates, Warren et al. ), because binding of EBNA1 to a pair of sites in DS or Rep* converts that DNA into an origin of DNA synthesis (Gahn and Schildkraut ), (Wang, Lindner et al.

The Epstein-Barr nuclear antigen 1 (EBNA1) protein enables the stable persistence of Epstein-Barr virus episomal genomes during latent infection, in part by tethering the episomes to the cellular chromosomes in mitosis.

A host nucleolar protein, EBNA1-binding protein 2 (EBP2), has been shown to be important for interactions between EBNA1 and chromosomes in metaphase and to associate with. chimeric antigen receptor (CAR) based on the monoclonal antibody TÜ which recognizes an Epstein-Barr nuclear antigen (EBNA)−3C-derived peptide in HLA-B*35 context in a T-cell receptor (TCR)-like manner.

In order to attract additional immune cells to proximity of PTLD cells, based on the TÜ CAR, we moreover generated. She also ordered a full thyroid panel (also normal) and a test for Epstein-Barr virus antibodies.

Description Reconstitution of Epstein-Barr nuclear antigen 1 (EBNA1)-mediated plasmid segregation in budding yeast requires human EBP2 EPUB

My levels are: EBV IgM is less t and EBV Nuclear Antigen, IgG. Infectious diseases are linked to 15%–20% of cancers worldwide. Among them, Epstein–Barr virus (EBV) is an oncogenic herpesvirus that chronically infects over 90% of the adult population, with overcases of cancer andcancer-related deaths attributed to it yearly.

Acute EBV infection can present as infectious mononucleosis, and lead to the future onset of multiple cancers. Epstein-Barr Nuclear Antigen 1 (EBNA1) is a multifunctional protein encoded by EBV.

EBNA1’s role in maintaining EBV in latently proliferating cells, by mediating EBV genome synthesis and nonrandom partitioning to daughter cells, as well as regulating viral gene transcription, is well characterized.

Details Reconstitution of Epstein-Barr nuclear antigen 1 (EBNA1)-mediated plasmid segregation in budding yeast requires human EBP2 FB2

Less understood are the roles of EBNA1 in affecting the host cell to provide selective. The Epstein–Barr virus (EBV) nuclear antigen (EBNA) 1 is the only viral protein regularly detected in all EBV-associated malignancies and is likely to be expressed also in latently infected B lymphocytes that serve as the viral reservoir in healthy virus carriers (reviewed in ref.

Several lines of. A far-reaching study conducted by scientists at Cincinnati Children's reports that the Epstein-Barr virus (EBV)—best known for causing mononucleosis—also increases. The Epstein-Barr virus is a type of herpes virus. It is very common, and because the symptoms are quite general, a doctor may order an Epstein-Barr virus blood test to.

Epstein-Barr virus (EBV)–encoded nuclear antigen (EBNA)1 is thought to escape cytotoxic T lymphocyte (CTL) recognition through either self-inhibition of synthesis or by blockade of proteasomal degradation by the glycine-alanine repeat (GAr) domain. Here we show that EBNA1 has a remarkably varied cell type–dependent stability.

The Epstein-Barr virus (EBV) nuclear antigen (EBNA)1 contains a glycine-alanine repeat (GAr) domain that appears to protect the antigen from proteasomal breakdown and, as measured in cytotoxicity assays, from major histocompatibility complex (MHC) class I–restricted presentation to CD8 + T cells.

This led to the concept of EBNA1 as an immunologically silent protein that although unique. The Epstein–Barr virus (EBV) nuclear antigen (EBNA)-1 promotes the accumulation of chromosomal aberrations in malignant B cells by inducing oxidative stress.

Here we .Sample J, Brooks L, Sample C, Young L, Rowe M, Gregory C, Rickinson A, Kieff E. Restricted Epstein-Barr virus protein expression in Burkitt lymphoma is due to a different Epstein-Barr nuclear antigen 1 transcriptional initiation site.

Proc Natl Acad Sci U S A. .Steroid sensitive nephrotic syndrome is marked by a massive proteinuria and loss of podocytes foot processes.

The mechanism of the disease remains debated but recent publications suggest a primary role of Epstein-Barr Virus (EBV). EBV replication in the peripheral blood is found in 50% of patients d .